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Which technique helped in the investigation of the Calvin cycle?

(a) X-ray technique Your answer
(b) X-ray crystallography Your answer
(c) intermittent light Your answer
(d) radioactive isotope technique Correct

Your answer: Correct answer: (d)

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Explanation

Why (d) is correct

Calvin traced the path of carbon in photosynthesis with radioactive ¹⁴C (an isotope), finding which compounds became labelled first.

Why the other options are wrong

(a) · your answer X-rays were not used to trace carbon in the Calvin cycle.
(b) · your answer X-ray crystallography studies the structure of molecules, not metabolic pathways.
(c) · your answer Intermittent light was used in other studies of the light reaction, not to trace the Calvin cycle.

NCERT point: Using ¹⁴CO₂, Calvin found that the first product of fixation was 3-PGA.

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